Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Multi-Mode Microplate Reader, Absorbance/Fluorescence/Luminescence, 6-1536 Well, Temperature Controlled
The SpectraMax i3x Multi-Mode Microplate Reader is a configurable, application-upgradeable microplate reader that performs absorbance, fluorescence, and luminescence assays across a broad range of plate formats. It provides broad wavelength coverage, high sensitivity, and modular detection options for diverse biochemical and cell-based workflows.
- Measures absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization.
- Wavelength coverage suitable for UV-vis and fluorescence assays.
- Xenon flash lamp and LED illumination options for flexible excitation.
- Supports 6-1536 well plates, including standard 96 and 384 formats.
- Top and bottom read capabilities with bottom read available for fluorescence assays.
- Temperature control from ambient +4 °C up to 45 °C, with optional shaker and injector configurations.
- Includes dedicated assay control and analysis software.
Molecular Devices SpectraMax i3x Microplate Reader, Multi-Mode, 6-1536 Well
The SpectraMax i3x is a configurable multi-mode microplate reader designed for biochemical and cell-based assays. It provides absorbance, fluorescence, and luminescence detection across a broad wavelength range, supports 6-1536 well plates, and can be field-upgraded with imaging, TRF/HTRF, AlphaScreen, injectors, and western blot detection modules.
- Configurable multi-mode detection for absorbance, fluorescence, and luminescence.
- Supports plate formats from 6 to 1536 wells.
- Broad wavelength coverage from UV to near-infrared.
- Field-upgradeable modules for imaging, TRF/HTRF, AlphaScreen, injectors, and western blot detection.
- High-sensitivity optics with xenon flash lamp and LED illumination options.
- Compact footprint suitable for bench or automated workflows.
Molecular Devices Multi-Mode Microplate Reader, Five-Mode, Dual Injectors, 6-384-Well
The SpectraMax iD5 multi-mode microplate reader with dual injectors provides flexible detection for absorbance, fluorescence, luminescence, and time-resolved assays, and enables injector-based kinetic workflows for rapid reagent delivery across plate formats.
- Five detection modes: absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization.
- Dual injectors with low dead volume and overflow protection for kinetic assays.
- Supports plate formats from 6 to 384 wells with well scanning up to 20 x 20.
- Absorbance range 230-1000 nm; fluorescence excitation 250-830 nm, emission 270-850 nm.
- Temperature control to 66 °C and linear, orbital, and double-orbital shaking.
- Compatible with data acquisition software and robotic integration for automated workflows.
REFEYN INC MP Auto - Alignment Kit, Package to affix the sample well cassette precisely and reliably to the sample carrier slide.
Alignment Assist Package to affix the sample well cassette precisely and reliably to the sample carrier slide. This includes: - MP Auto alignment tool - Carbon fibre tip tweezers- Set of magnets (4)
